Posted on April 18, 2019

ASTM E2149 PDF

ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.

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The number of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.

Record and report presence of solution activity. This test method is designed to evaluate the antimicrobial activity of non-leaching, antimicrobial-treated specimens under dynamic contact conditions.

The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time.

Test Package Request Form. Microbial concentrations in the treated and untreated samples are usually determined at 0, 1 or 24 hours contact time. Facebook Linkedin Instagram Twitter Google. Link to Active This link will always route to the current Active version of the standard. To determine if a compound is immobilized in all conditions or during the end use of the product additional testing may be required.

Immobilized antimicrobial agents, such as surface bonded materials, are not free to diffuse into their environment under normal conditions of use. It is for this reason that the aztm of the term leaching throughout this aatm is limited to only the testing conditions described herein.

Following exposure, a sample of the test organism suspension is removed quantitatively assayed for survivors. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM E2149 Antimicrobial Activity

The flask is placed onto a wrist-action shaker and shaken for a desired exposure time, typically 1 hour. The resulting asmt are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension. Historical Version s – view previous versions of standard.

F2149 test method is only intended to determine efficacy as described in 4. No regulated reduction limits currently exist for general antimicrobial claims made using this method.

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Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard. These cookies collect information about how you interact with our website and allow us to remember you.

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It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Presence of residual antimicrobial activity indicates the presence of a leaching type of 2e149 agent. Maria Curry ext Wrist Ashm Shaker. Active view current version of standard.

It has been developed for routine quality control and screening tests and is used to evaluate substrate bound antimicrobials.

This test method is designed to evaluate the resistance of specimens treated with a non-leaching antimicrobial agent to the growth of microbes under dynamic contact conditions. Note 1 — Stresses may include laundry, wear and abrasion, radiation and steam sterilization, UV exposure, solvent manipulation, temperature susceptibility, or similar physical or chemical manipulation. This standard does not purport to address all of the safety concerns, if any, associated with its use.

ASTM E – Method Determining the Antimicrobial Activity

These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.

This standard does not purport to address all of the safety concerns, if any, associated with its use. This website stores cookies on your computer.

Work Item s – proposed revisions of this standard. Summary of Test In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli.

For example, water soluble antimicrobials will be prone to removal from the test surface using the method described in Section 13 but insoluble compounds will not. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.

Link to Active This link will always route to the current Active version of the standard. This test method ensures good contact between the bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the test specimen in a challenge suspension during the test period.

The presence of a leaching antimicrobial is determined post-test. One-hour contact time in a buffer solution allows for metabolic stasis in the population. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism.

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Work Item s – proposed revisions of this standard. We use this information to improve and customize your browsing experience and for analytics and metrics about our visitors — both on this website and other media. Surface antimicrobial activity is determined by comparing results from the test sample to controls run simultaneously.

This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials. This test ensures good contact between bacteria and the treated fibre by constant agitation of the test specimen in a bacterial suspension during the test period 1hour.

All the samples are shaken in dynamic shake flasks in a wrist action shaker to ensure good contact between the bacteria and the treated fibre, fabric, or other substrate by constant agitation of the test specimen in a bacterial suspension during the test period. Accept Decline Privacy policy. The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent is dependent upon direct contact of microbes with the active chemical agent.

To find out more about the cookies we use, see our Privacy Policy. The ASTM E Standard Test Method is used to evaluate the antimicrobial activity of substrate-bound, non-leaching, antimicrobial-treated samples under dynamic contact conditions. This dynamic shake flash test was developed for routine quality control and screening test.

Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard. Materials evaluated by this method may be comprised of fabric, paper, powder or other solid materials.

The susceptibility of the species to particular biocides could be altered depending on its life stage cycle. If an antimicrobial agent that is shown to be removed from aatm surface by Section 13 is utilized in this test methodology, controls must be included such that appropriate neutralization steps are including during recovery and enumeration.

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